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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of <t>DNA</t> <t>substrates</t> from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.
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Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of DNA substrates from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Establishment of the first Chinese national standard for recombinant nuclease used as a process material in pharmaceutical manufacturing

doi: 10.3389/fbioe.2025.1695176

Figure Lengend Snippet: Establishment of substrate quality standards for the activity determination method. (A) Nucleic acid electrophoresis results of DNA substrates from three manufacturers. Lanes 1–3: DNA substrate from Solarbio; Lanes 4–6: DNA substrate from Invitrogen; Lanes 7–9: DNA substrate from Promega; M: marker. (B) Linear equations fitted by plotting ΔOD 260 against time for reactions between RSN and DNA substrates from three manufacturers, n = 3. (C) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Invitrogen at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted DNA substrate control. (D) Nucleic acid electrophoresis results of reactions between RSN and DNA substrate from Solarbio at different times. Lanes 1–6: 5, 15, 30, 45, 60, and 90 min, respectively; Lane 7: unreacted control.

Article Snippet: Preliminary studies showed differences in activity when the candidate reference material was measured using herring sperm DNA substrates from three common brands (Invitrogen, Promega, and Solarbio), as described in .

Techniques: Activity Assay, Nucleic Acid Electrophoresis, Marker, Control